April 22, 2015

Bionanotechnology and Microbiology Lab

José Manuel Pérez-Donoso

Research Professor  
jose.perez@unab.cl
+56-2-770 3629

In the BioNanotechnology and Microbiology lab we are interested in studying the biological mechanisms involved in bacterial biosynthesis of metal nanoparticles (Cu, Li, Cd, Te, Se, Ag, In, among others), with special emphasis in fluorescent semiconductor nanoparticles (quantum dots). One of our research goals is to understand the biomineralization process that allows the bioproduction of nanocrystals of Biotechnological interest in bacterial strains inhabiting different extreme environments (Antarctica, Atacama desert, Salar flats, Dead Sea, Volcanoes, Mining sites, Uyuny salar, among others).

We are also interested in developing new eco-friendly biochemical methods for the production of nanoparticles based on the biomolecules or synthetic conditions determined in bacterial biosynthesis (“biomimetic” methods).

In addition, part of our fundamental research focuses on generate biotechnological applications using our biological or biomimetic nanoparticles, such us; manufacturing of third and fourth generation solar cells (QDs Sensitized Solar Cells and “Biological Solar Cells”), fluorescent detection of specific antigens (multiplexing), detection of Cu (nM) in complex samples such as bacterial growth media, to develop bioremediation strategies of heavy metals and hydrocarbons, cell targeting in biomedicine, generation of next generation batteries based on Li nanoparticles, photodynamic therapy, use of nanoparticles as antimicrobial agents, among many others.

Our laboratory have many active scientific collaborations with laboratories and research centers from EE.UU., Perú, Ireland, Argentina, Brazil, Canada and UK, among others. Due to these partnerships our students have the opportunity to perform short research stays, as well as conducting scientific expeditions to extreme environments for isolating new microorganisms.

In addition, we work in strong collaboration with different foundations and biotech companies.

 

Publications

2018


 TITLEAUTHORSJOURNAL
2Arsenic and Cadmium Bioremediation by Antarctic Bacteria Capable of Biosynthesizing CdS Fluorescent NanoparticlesDaniel A. Glatstein, Ph.D.; Nicolás Bruna; Carla Gallardo-Benavente; Denisse Bravo, Ph.D.;Magalí E. Carro Pérez, Ph.D.; Franco M. Francisca, Ph.D.; and  José M. Pérez-Donoso, Ph.D.Journal of Environmental Engineering. 2018. DOI: 10.1061/(ASCE)EE.1943-7870.0001293.
6Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidansATCC 19703 by Improving Metal Uptake and ToleranceGiovanni Ulloa, Carolina P. Quezada, Mabel Araneda , Blanca Escobar , Edwar Fuentes, Sergio A. Álvarez, Matías Castro, Nicolás Bruna, Rodrigo Espinoza-González, Denisse Bravo and José M. Pérez-Donoso .Frontiers in Microbiology. 2018. DOI: 10.3389/fmicb.2018.00234
11Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidansATCC 19703 by Improving Metal Uptake and ToleranceGiovanni Ulloa, Carolina P. Quezada, Mabel Araneda, Blanca Escobar, Edwar Fuentes, Sergio A. Álvarez, Matías Castro, Nicolás Bruna, Rodrigo Espinoza-González, Denisse Bravo and  José M. Pérez-Donoso.Frontiers Microbiology. 2018 DOI.: 10.3389/fmicb.2018.00234

2017


 TITLEAUTHORSJOURNAL
107Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440.Oliva-Arancibia B, Órdenes-Aenishanslins N,  Bruna N , Ibarra PS, Zacconi FC,  Pérez-Donoso JM, Poblete-Castro I. Journal of Biotechnology. 2017. DOI: 10.1016/j.jbiotec.2017.10.013
105A comparative analysis of tellurite detoxification by members of the genus Shewanella.Valdivia-González M., Díaz-Vásquez W, Ruiz-León, Becerra A,  Aguayo D ,  Pérez-Donoso JM , Vásquez C. Archives of Microbiology. 2017. DOI: 10.1007/s00203-017-1438-2
96Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiolsF. A. Venegas,  L. A. Saona,  J. P. Monrás,  N. Órdenes-Aenishanslins,  M. F. Giordana,  G. Ulloa,  B. Collao,  D. Bravod  and   J. M. Pérez-Donoso.Royal Society of Chemistry. 2017. DOI: 10.1039/C7RA03578K.
95Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils.Gran-Scheuch, Fuentes E, Bravo DM, Jiménez JC,  Pérez-Donoso JM .Frontiers in Microbiology. 2017. DOI: 10.3389/fmicb.2017.01634

2016


 TITLEAUTHORSJOURNAL
55The Porphyromonas gingivalis O antigen is required for inhibition of apoptosis in gingival epithelial cells following bacterial infectionSoto C., Bugueño I., Hoare A., Gonzalez S., Venegas D., Salinas D., Melgar-Rodríguez S., Vernal R., Gamonal J., Quest AF., Pérez-Donoso JM., Bravo D.Journal of Periodontal Research. 2016. DOI: 10.1111/jre.12331
40Use of acidophilic bacteria of the genus Acidithiobacillus to biosynthesize CdS fluorescent nanoparticles (quantum dots) with high tolerance to acidic pHUlloa G, Collao B, Araneda M., Escobar B., Álvarez S., Bravo D., Pérez-Donoso JM..Enzyme Microb Technol. 2016. DOI: 10.1016/j.enzmictec.2016.09.005.

2015


 TITLEAUTHORSJOURNAL
26Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistanceDíaz L, Hoare A, Soto C, Bugueño I, Silva N, Dutzan N, Venegas D, Salinas D, Pérez-Donoso JM, Gamonal J, Bravo D.Anaerobe. 2015. DOI: 10.1016/j.anaerobe.2015.01.009
22Thermal- and photo-stability of glutathione-capped cadmium telluride Quantum DotsWansapura P., Díaz-Vásquez W., Vásquez C., Pérez-Donoso J.M. and Chasteen TJournal of Applied BioMaterials and Functional Materials. 2015. DOI: 10.5301/jabfm.5000221
11The Porphyromonas gingivalis O-antigen is required for inhibition of apoptosis in gingival epithelial cells following bacterial infection.C. Soto, I. Bugueño, A. Hoare, S. Gonzalez, D. Venegas, D. Salinas, S. Melgar-Rodríguez, R. Vernal, J. Gamonal, A. F. G. Quest, J. M. Pérez-Donoso, D. BravoJournal of Periodontal Research.2015. DOI: 10.1111/jre.12331.

2014


 TITLEAUTHORSJOURNAL
9Quantum dot-based assay for Cu2+ quantification in bacterial cell cultureV. Durán-Toroa, A. Gran-Scheucha, N. Órdenes-Aenishanslinsa, J.P. Monrása, L.A. Saonaa, F.A. Venegasa, T.G. Chasteend, D. Bravoe, J.M. Pérez-Donoso.Analytical Biochemistry. 2014. DOI: 10.1016/j.ab.2014.01.001
8Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from AntarcticaArenas, Felipe A.; Pugin, Benoit; Henriquez, Nicole A.; José M. Pérez-Donoso.Polar Science. 2014. DOI: 10.1016/j.polar.2014.01.001.

2013


 TITLEAUTHORSJOURNAL

2012


 TITLEAUTHORSJOURNAL


 

Patents

  • Pérez-Donoso J.M.*, Saona L. y Órdenes-Aenishanslins N. (2014) “Síntesis química de nanopartículas de sulfuro de cobre”. International application number: PCT/CL2014/000073, Fecha presentación 10/12/14, país Chile.
  • Pérez-Donoso J.M.* y Saona L. (2014) “Método microbiológico para producir nanopartículas de sulfuro de cobre”. International application number: PCT/CL2014/000074, Fecha presentación 10/12/14, país Chile.
  • Pérez-Donoso J.M.*, Venegas F. y Monrás J.P. (2014) “Síntesis acuosa de nanopartículas de sulfuro de cadmio”. International application number: PCT/CL2014/000075. Fecha presentación 10/12/14, país Chile.
  • Pérez-Donoso J.M.*, Monrás J. P., Osorio-Román I.O. y Vásquez C.C. (2013) “Synthesis of highly fluorescent GSH CdTe nanoparticles (quantum dots)”. US patent application, publication number US20130284979 A1, application number: US 13/929,484, publication date 31/10/13, país USA.
  • Pérez-Donoso J.M.*, Monrás J. P., Osorio-Román I.O. y Vásquez C.C. (2012) “Synthesis of highly fluorescent GSH CdTe nanoparticles (quantum dots)”. World International Property Association (WIPO), publication number WO/2012/090161, international application number PCT/IB2011/055976, publication date 05/07/2012, international filing date 27/12/2011.