April 22, 2015

Bionanotechnology and Microbiology Lab

José Manuel Pérez-Donoso

Research Professor  
+56-2-770 3629

In the BioNanotechnology and Microbiology lab we are interested in studying the biological mechanisms involved in bacterial biosynthesis of metal nanoparticles (Cu, Li, Cd, Te, Se, Ag, In, among others), with special emphasis in fluorescent semiconductor nanoparticles (quantum dots). One of our research goals is to understand the biomineralization process that allows the bioproduction of nanocrystals of Biotechnological interest in bacterial strains inhabiting different extreme environments (Antarctica, Atacama desert, Salar flats, Dead Sea, Volcanoes, Mining sites, Uyuny salar, among others).

We are also interested in developing new eco-friendly biochemical methods for the production of nanoparticles based on the biomolecules or synthetic conditions determined in bacterial biosynthesis (“biomimetic” methods).

In addition, part of our fundamental research focuses on generate biotechnological applications using our biological or biomimetic nanoparticles, such us; manufacturing of third and fourth generation solar cells (QDs Sensitized Solar Cells and “Biological Solar Cells”), fluorescent detection of specific antigens (multiplexing), detection of Cu (nM) in complex samples such as bacterial growth media, to develop bioremediation strategies of heavy metals and hydrocarbons, cell targeting in biomedicine, generation of next generation batteries based on Li nanoparticles, photodynamic therapy, use of nanoparticles as antimicrobial agents, among many others.

Our laboratory have many active scientific collaborations with laboratories and research centers from EE.UU., Perú, Ireland, Argentina, Brazil, Canada and UK, among others. Due to these partnerships our students have the opportunity to perform short research stays, as well as conducting scientific expeditions to extreme environments for isolating new microorganisms.

In addition, we work in strong collaboration with different foundations and biotech companies.



In Silico Study of Coumarins and Quinolines Derivatives as Potent Inhibitors of SARS-CoV-2 Main ProteaseOsvaldo Yañez, Manuel Isaías Osorio, Eugenio Uriarte, Carlos Areche, William Tiznado, José M. Pérez-Donoso , Olimpo García-Beltrán, Fernando González-NiloFrontiers in Chemistry. 2021. https://doi.org/10.3389/fchem.2020.595097
Theobroma cacao L. compounds: Theoretical study and molecular modelling as inhibitors of main SARS-CoV-2 proteaseOsvaldo Yañez, Manuel Isaías Osorio, Carlos Areche, Alejandro Vasquez-Espinal, Jessica Bravo, Angélica Sandoval-Aldana, José M Pérez-Donoso , Fernando González-Nilo , Maria João Matos, Edison Osorio, Olimpo García-Beltrán, William TiznadoBiomedicine & Pharmacotherapy. 2021. https://doi.org/10.1016/j.biopha.2021.111764
Production of cadmium sulfide quantum dots by the lithobiontic Antarctic strain Pedobacter sp. UYP1 and their application as photosensitizer in solar cellsV. Carrasco, V. Amarelle, S. Lagos-Moraga, C. P. Quezada, R. Espinoza-González, R. Faccio, E. Fabiano & J. M. Pérez-Donoso Microbial Cell Factories. 2021. https://doi.org/10.1186/s12934-021-01531-4
Biomimetic synthesis of CuInS2 nanoparticles: characterization, cytotoxicity, and application in quantum dots sensitized solar cellsC Arriaza-Echanes, JL Campo-Giraldo, CP Quezada, R Espinoza-González, P Rivas-Álvarez, M Pacheco, D Bravo, JM Pérez-DonosoArabian Journal of Chemistry. 2021. https://doi.org/10.1016/j.arabjc.2021.103176
Genomics Insights into Pseudomonas sp. CG01: An Antarctic Cadmium-Resistant Strain Capable of Biosynthesizing CdS Nanoparticles Using Methionine as S-SourceCarla Gallardo-Benavente, Jessica L Campo-Giraldo, Juan Castro-Severyn, Andrés Quiroz, José M Pérez-DonosoGenes. 2021. https://doi.org/ 10.3390/genes12020187


Draft Genome Sequence of Blautia luti DSM 14534T, Isolated from Human StoolRodrigo L. Ortiz, Felipe Melis-Arcos, Paulo C. Covarrubias, Juan A. Ugalde, Zachary S. Apte, José Pérez-Donoso , Juan P. Cárdenas, Daniel E. AlmonacidMicrobioly Resource Announcements, 2020. DOI: 10.1128/MRA.00088-20
Bacterial Synthesis of Ternary CdSAg Quantum Dots through Cation Exchange: Tuning the Composition and Properties of Biological Nanoparticles for Bioimaging and Photovoltaic ApplicationsNicolás Órdenes-Aenishanslins, Giovanna Anziani-Ostuni, Juan Pablo Monrás, José Manuel Pérez-Donoso , et al.Microorganisms. 2020. DOI: 10.3390/microorganisms8050631
Effect of Co-contamination by PAHs and Heavy Metals on Bacterial Communities of Diesel Contaminated Soils of South Shetland Islands, AntarcticaAlejandro Gran-Scheuch, Javiera Ramos-Zuñiga, Edwar Fuentes, Denisse Bravo, José M Pérez-DonosoMicroorganisms. 2020. https://doi.org/10.3390/microorganisms8111749


Synthesis of salt-stable fluorescent nanoparticles (quantum dots) by polyextremophile halophilic bacteria.N. Bruna, B. Collao, A. Tello, P. Caravantes, N. Díaz-Silva, J. P. Monrás, N. Órdenes-Aenishanslins, M. Flores, R. Espinoza-Gonzalez, D. Bravo & J. M. Pérez-Donoso Scientific Report. https://doi.org/10.1038/s41598-018-38330-8
Variability in Genomic and Virulent Properties of Porphyromonas gingivalis Strains Isolated From Healthy and Severe Chronic Periodontitis IndividualsKatterinne N. Mendez, Anilei Hoare, Cristopher Soto, Isaac Bugueño, Marcela Olivera, Claudio Meneses, Jose Manuel Pérez-Donoso, Eduardo Castro-Nallar and Denisse Bravo.Fontier in Cellular and Infection Microbiology. 2019. doi.org/10.3389/fcimb.2019.00246
Biological Synthesis of CdS/CdSe Core/Shell Nanoparticles and Its Application in Quantum Dot Sensitized Solar CellsNicolás Órdenes-Aenishanslins, Giovanna Anziani-Ostuni, Carolina P. Quezada, Rodrigo Espinoza-González, Denisse Bravo and José M. Pérez-DonosoFrontier in Micronbiology. 2019. doi.org/10.3389/fmicb.2019.01587
Biosynthesis of CdS Quantum Dots Mediated by Volatile Sulfur Compounds Released by Antarctic Pseudomonas fragiCarla Gallardo-Benavente, Ornella Carrión, Jonathan D. Todd, Joana C. Pieretti, Amedea B. Seabra, Nelson Durán, Olga Rubilar, José M. Pérez-Donoso and Andrés Quiroz.Frontier in Microbiology. 2019. doi.org/10.3389/fmicb.2019.01866
Odd Loop Regions of XenA and XenB Enzymes Modulate Their Interaction with Nitro-explosives Compounds and Provide Structural Support for Their RegioselectivityManuel I. Osorio, Ma. Angeles Cabrera, Fernando González-Nilo, José M. Pérez-Donoso.Journal of Chemical Information and Modeling. 2019. DOI: 10.1021/acs.jcim.9b00357


113Arsenic and Cadmium Bioremediation by Antarctic Bacteria Capable of Biosynthesizing CdS Fluorescent NanoparticlesDaniel A. Glatstein, Ph.D.; Nicolás Bruna; Carla Gallardo-Benavente; Denisse Bravo, Ph.D.;Magalí E. Carro Pérez, Ph.D.; Franco M. Francisca, Ph.D.; and José M. Pérez-Donoso, Ph.D.Journal of Environmental Engineering. 2018. DOI: 10.1061/(ASCE)EE.1943-7870.0001293.
117Phosphate Favors the Biosynthesis of CdS Quantum Dots in Acidithiobacillus thiooxidansATCC 19703 by Improving Metal Uptake and ToleranceGiovanni Ulloa, Carolina P. Quezada, Mabel Araneda , Blanca Escobar , Edwar Fuentes, Sergio A. Álvarez, Matías Castro, Nicolás Bruna, Rodrigo Espinoza-González, Denisse Bravo and José M. Pérez-Donoso.Frontiers in Microbiology. 2018. DOI: 10.3389/fmicb.2018.00234
137 Biomimetic quantum dot-labeled B16F10 murine melanoma cells as a tool to monitor early steps of lung metastasis by in vivo imaging. International Journal of NanomedicineDíaz-García V.M, Guerrero S, Díaz N, Lobos-González L, Catalán J, Kogan M. J, Pérez-Donoso J. M and Quest A.F.GInternational Journal of Nanomedicine. 2018.


107Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440.Oliva-Arancibia B, Órdenes-Aenishanslins N,  Bruna N , Ibarra PS, Zacconi FC,  Pérez-Donoso JM, Poblete-Castro I. Journal of Biotechnology. 2017. DOI: 10.1016/j.jbiotec.2017.10.013
105A comparative analysis of tellurite detoxification by members of the genus Shewanella.Valdivia-González M., Díaz-Vásquez W, Ruiz-León, Becerra A,  Aguayo D ,  Pérez-Donoso JM , Vásquez C. Archives of Microbiology. 2017. DOI: 10.1007/s00203-017-1438-2
96Biological phosphorylated molecules participate in the biomimetic and biological synthesis of cadmium sulphide quantum dots by promoting H2S release from cellular thiolsF. A. Venegas,  L. A. Saona,  J. P. Monrás,  N. Órdenes-Aenishanslins,  M. F. Giordana,  G. Ulloa,  B. Collao,  D. Bravod  and   J. M. Pérez-Donoso.Royal Society of Chemistry. 2017. DOI: 10.1039/C7RA03578K.
95Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils.Gran-Scheuch, Fuentes E, Bravo DM, Jiménez JC,  Pérez-Donoso JM .Frontiers in Microbiology. 2017. DOI: 10.3389/fmicb.2017.01634


55The Porphyromonas gingivalis O antigen is required for inhibition of apoptosis in gingival epithelial cells following bacterial infectionSoto C., Bugueño I., Hoare A., Gonzalez S., Venegas D., Salinas D., Melgar-Rodríguez S., Vernal R., Gamonal J., Quest AF., Pérez-Donoso JM., Bravo D.Journal of Periodontal Research. 2016. DOI: 10.1111/jre.12331
40Use of acidophilic bacteria of the genus Acidithiobacillus to biosynthesize CdS fluorescent nanoparticles (quantum dots) with high tolerance to acidic pHUlloa G, Collao B, Araneda M., Escobar B., Álvarez S., Bravo D., Pérez-Donoso JM..Enzyme Microb Technol. 2016. DOI: 10.1016/j.enzmictec.2016.09.005.


26Changes in lipopolysaccharide profile of Porphyromonas gingivalis clinical isolates correlate with changes in colony morphology and polymyxin B resistanceDíaz L, Hoare A, Soto C, Bugueño I, Silva N, Dutzan N, Venegas D, Salinas D, Pérez-Donoso JM, Gamonal J, Bravo D.Anaerobe. 2015. DOI: 10.1016/j.anaerobe.2015.01.009
22Thermal- and photo-stability of glutathione-capped cadmium telluride Quantum DotsWansapura P., Díaz-Vásquez W., Vásquez C., Pérez-Donoso J.M. and Chasteen TJournal of Applied BioMaterials and Functional Materials. 2015. DOI: 10.5301/jabfm.5000221
11The Porphyromonas gingivalis O-antigen is required for inhibition of apoptosis in gingival epithelial cells following bacterial infection.C. Soto, I. Bugueño, A. Hoare, S. Gonzalez, D. Venegas, D. Salinas, S. Melgar-Rodríguez, R. Vernal, J. Gamonal, A. F. G. Quest, J. M. Pérez-Donoso, D. BravoJournal of Periodontal Research.2015. DOI: 10.1111/jre.12331.


9Quantum dot-based assay for Cu2+ quantification in bacterial cell cultureV. Durán-Toroa, A. Gran-Scheucha, N. Órdenes-Aenishanslinsa, J.P. Monrása, L.A. Saonaa, F.A. Venegasa, T.G. Chasteend, D. Bravoe, J.M. Pérez-Donoso.Analytical Biochemistry. 2014. DOI: 10.1016/j.ab.2014.01.001
8Isolation, identification and characterization of highly tellurite-resistant, tellurite-reducing bacteria from AntarcticaArenas, Felipe A.; Pugin, Benoit; Henriquez, Nicole A.; José M. Pérez-Donoso.Polar Science. 2014. DOI: 10.1016/j.polar.2014.01.001.