April 22, 2015

Biosystems Engineering Lab

Ignacio Poblete-Castro

Assistant Professor   
+56-2-2770 3625

 Our group focuses on engineering biological systems for the synthesis of value-added chemical compounds. Among them, we center our attention to the production of biodegradable polymers, biofuels, proteins, and organic acids. Driven by a systems biotechnological approach we also pursue to develop new bioprocesses using waste materials as carbon substrates, where the host strain is modulated (rewire) and coupled to the process to improve performance in bioreactors. In addition, we also attempt to systematically understand microbial metabolism (single and communities) via metabolic network reconstruction and simulation. 



In-Depth Genomic and Phenotypic Characterization of the Antarctic Psychrotolerant Strain Pseudomonas sp. MPC6 Reveals Unique Metabolic Features, Plasticity, and Biotechnological PotentialMatias Orellana-Saez, Nicolas Pacheco, José I. Costa, Katterinne N. Mendez, Matthieu J. Miossec , Claudio Meneses, Eduardo Castro-Nallar , Andrés E. Marcoleta, Ignacio Poblete-Castro.Frontier in Micronbiology. 2019. doi.org/10.3389/fmicb.2019.01154
Exploiting the natural poly(3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymersNicolas Pacheco, Matias Orellana-Saez, Marzena Pepczynska, Javier Enrione, Monica Bassas-Galia, Jose M Borrero-de Acuña, Flavia C Zacconi, Andrés E Marcoleta, Ignacio Poblete-CastroJournal of industrial microbiology & biotechnology. 2019. doi.org/10.1007/s10295-019-02186-2


122Genome sequence of two members of the chloroaromatic-degrading MT community: Pseudomonas reinekei MT1 and Achromobacter xylosoxidans MT3.Gutierrez-Urrutia I, Miossec MJ, Valenzuela SL, Meneses C, Dos Santos VAPM, Castro-Nallar E, Poblete-Castro I.Journal of Biotechnology. 2018. DOI: 10.1016/j.jbiotec.2018.03.019.
138Enhanced synthesis of medium-chain-length Poly(3-hydroxyalkanoates) by inactivating the tricarboxylate transport system of Pseudomonas putida KT2440 and process development using waste vegetable oilJosé ManuelBorrero-de Acuña, Carla Aravena-Carrasco, IzabookGutierrez-Urrutia, Daniela Duchens, IgnacioPoblete-CastroProcess Biochemistry. 2018. doi.org/10.1016/j.procbio.2018.10.012


107Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida KT2440.Oliva-Arancibia B, Órdenes-Aenishanslins N,  Bruna N , Ibarra PS, Zacconi FC,  Pérez-Donoso JM, Poblete-Castro I. Journal of Biotechnology. 2017. DOI: 10.1016/j.jbiotec.2017.10.013
101A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.Borrero-de Acuña JM, Hidalgo-Dumont,Pacheco N, Cabrera A,  Poblete-Castro I. Scientific Reports. 2017 DOI: 10.1038/s41598-017-04741-2.
74Multi-level evaluation of Escherichia coli polyphosphate related mutants using global transcriptomic, proteomic and phenomic analyses.Varas M, Valdivieso C, Mauriaca C, Ortíz-Severín J, Paradela A,  Poblete-Castro I , Cabrera R, Chávez FP.Biochemica et Biophysica acta. 2017. DOI: 10.1016/j.bbagen.2017.01.007
73Datasets for transcriptomics, q-proteomics and phenotype microarrays of polyphosphate metabolism mutants from Escherichia coli.Macarena Varas, Camilo Valdivieso, Cecilia Mauriaca,a Javiera Ortíz-Severín, Alberto Paradela,  Ignacio Poblete-Castro , Ricardo Cabrera, Francisco P. Cháveza.Data Brief. 2017. DOI: 10.1016/j.dib.2017.03.010
72Batch Cultivation Model for Biopolymer Production.C. E. Torres-Cerna, A. Y. Alanis,a, I. Poblete-Castro , E. A. Hernandez-Vargas.Chemical and Biochemical Engineering Quarterly. 2017. DOI: 10.15255/CABEQ.2016.952


53Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerolBeckers, V.; Poblete-Castro, I.; Tomasch, J.; Wittmann, C.Microbial Cell Factories. 2016, DOI: 10.1186/s12934-016-0470-2


15Draft Genome Sequence of the Phenol-Degrading Bacterium Pseudomonas putida H.Vizoso P, Pacheco N, Bastias-Molina M, Meneses C, Poblete-Castro I.Genome Announcement. 2015. DOI: 10.1128/genomeA.00936-15.